Use of cryopreserved spermatozoa for caprine in vitro fertilization (IVF)
نویسندگان
چکیده
منابع مشابه
STANDARD IVF In Vitro Fertilization, Fertilization
In vitro fertilization (IVF) is a process by which an egg is fertilised by sperm outside the body: in vitro . The process involves monitoring and stimulating a woman's ovulatory process, removing an ovum or ova (egg or eggs) from the woman's ovaries and letting sperm fertilise them in a liquid in a laboratory. The fertilised egg (zygote) is cultured for 2–6 days in a growth medium and is then i...
متن کاملIVF, in vitro fertilization, article
In its simplest term, IVF is simply the uniting of egg and sperm in vitro (in the lab). Subsequently the embryos are transferred into the uterus through the cervix and pregnancy is allowed to begin. IVF was the first of the ART techniques to be developed. The first birth was in 1978 in England. The procedure was pioneered by a Gynecologist and a Ph.D. (Drs. Steptoe and Edwards). Next came GIFT,...
متن کاملCaprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders.
The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM...
متن کاملA comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured caprine oocytes
The aim of the study was to compare the parthenogenetic activation and in vitro fertilization (IVF) of in vitro matured caprine oocytes. A total of 881 cumulus-oocyte complexes (COC’s) were collected from 243 ovaries. Oocytes were matured in TCM-199 medium containing eCG (20 IU/ml), hCG (20 IUμg/ml), oestradiol-17β (1 μg/ml), BSA embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...
متن کاملP-141: Comparison Study The Effect of In Vitro Maturation and IVF Fertilization of Cryopreserved Oocyte with Fresh Oocyte in Mice
Background: Cryobiology and in vitro maturation of oocytes are very important tools of reproductive technology. Oocyte plays an important role in fertility. The objective of this research is comparison study the effect of invitro maturation and IVF fertilization of cryopreserved oocyte with fresh oocyte in mice. Materials and Methods: For this purpose, after superovulation was performed oocytes...
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ژورنال
عنوان ژورنال: Theriogenology
سال: 1997
ISSN: 0093-691X
DOI: 10.1016/s0093-691x(97)82426-0